Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. Activate your 30 day free trialto unlock unlimited reading. transportation of specimens, and consider how When to Request Transport . 1. Think like a microbe. endstream objective. 5 0 obj Clipping is a handy way to collect important slides you want to go back to later. Check the requisition form for requested tests, patient information, and any special requirements. Our product offerings include millions of PowerPoint templates, diagrams, animated 3D characters and more. 4. Many drugs will have "peak" and "trough" levels that vary according to dosage levels and intervals. Avoid repeated freezing and thawing of specimens. Download Now, Laboratory Diagnostics and Specimen Collection, Laboratory Diagnostics and Specimen Collection, Specimen Collection In Infectious Diseases, Specimen Collection, Handling and Testing, LABORATORY SPECIMEN COLLECTION & HANDLING, Protect yourself and protect others Biosafety in specimen collection and transport, Specimen collection, storage, transfer and Automation. Throat Swab Culture 2. The essential elements of the requisition cont., Histopathology specimen request must contain in addition to the above listed information, the following: name of the procedure, no. Type of specimen, if two, mention as the first of second. The PowerPoint PPT presentation: "Sample collection, transport" is the property of its rightful owner. 3. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. nurses often assume the responsibility of specimen collection. Supplies. There is a clot activator present and inversion expedites the clotting process. Appropriate samples from the appropriate site and an adequate amount should be collected. Random Urine Sample 4.2. OF SPECIMENS In general, specimens for virus isolation should be collected within 4 days after onset of illness as virus shedding decreases rapidly after that time. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) Check for timing instructions giving by the physician for drawing the appropriate samples. specimen. Needles - The gauge number indicates the bore size: the larger the gauge number, the smaller the needle bore. Promptly send the specimens with the requisition to the laboratory. Role of specimen collection in infectious disease by Dr. T. V. Rao MD . The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) Specimens must be submitted to the laboratory in the container used originally for collection. It's FREE! xK@sLngf?`'MqXC~//0p: Fp1{!BDb2F)8^?Z(qDP~#:B^}tGzMAP|fzmF"ne`R_D>$"e_v`H1^Vge[ZH@zg$f =)Cu='o"pq.:89]mzM:YRl4OMy}y9(k{V5hm[!rw}A8q/g;`T],p*#Y\&}?il&8r;NF|D Details about immunization and antimicrobial therapy. Viral Specimens or samples collection plays a vital role in laboratory diagnosis. Collect the sample within 5 days of the onset of the symptoms, and the second sample 5-10 days later. Activate your 30 day free trialto continue reading. Do you have PowerPoint slides to share? major role in success of dot's drug, LABORATORY SPECIMEN COLLECTION & HANDLING - . Frozen specimens thawing during transport b. Unspun specimens transported >2 hours from collection Specimen Collection Patient results are only as good as the specimen collected. Serena Lobo Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. Skin specimens are required to investigate infections caused by herpes simplex viruses, rabies virus. Sample should be collected aseptically. This study provides information about the sales and revenue during the historic and forecasted period of 2015 to 2026. A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. Clinical staff a. specimen collection and transport. Some sex hormones need special timing as regard cycle : (1)FSH,LH (3rd day of cycal {follicular phase}) (2)Progesterone (21 day of cycle{ luteal phase}) Other Factors: Age, gender, and pregnancy have an influence on laboratory testing. It appears that you have an ad-blocker running. jitendra kumar pandey,mgm medical college,mumbai. Activate your 30 day free trialto continue reading. Errors Anticoagulant cross contamination Citrate : If drawing a blue top tube for coagulation tests, (other than PT and PTT), a red tube (2-3 cc) must be drawn first to avoid contamination from tissue thromboplastin, which can yield false coagulation Results. - Collection of Epidemiologic Data Bao-Ping Zhu, MD, MS - Title: Slide 1 Created Date: 11/20/2008 5:55:36 PM Document presentation format: On-screen Show (4:3) Other titles: Arial Majalla UI Calibri Traditional Arabic How Warring Medical Kits Provides Valued Protection for Specimen Collection. 5. Proper sample collection leads to the proper diagnosis of disease. The Phlebotomist/Courier transports laboratory specimens to and from the clinic site and Singing River Health System in accordance with departmental and System procedures and guidelines. Processing of urine in microbiology by Rahul raj, Cytopathology Lab manual for MLT Students, Abnormalities of the Pupil copy copy copy.pptx, Westside Wellness - Mobile IV Hydration Therapy, Intensification Options after basal Insulin Revisited, health related behaviour ( ).pptx, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Special specimen collection procedures, including more invasive procedures, or those with an increased risk of complications to the patient, (e.g. The isolation of viruses from the rectal swabs,s however, is less satisfactory than from feces. Patients Preparation factors Therapeutic Drug Monitoring: different pharmacologic agents have patterns of administration, body distribution, metabolism, and elimination that affect the drug concentration as measured in the blood. The SlideShare family just got bigger. We've updated our privacy policy. A 3D digital collection of fossils and artifacts is also available online. foundation block (2018). Be aware that the microbes, Good microbiology cannot be performed on a bad, Surface cleansing with alcohol and iodine for, Rinsing oral cavity with water before collecting, Touching only inflamed area of the throat, Cleansing the external genitalia before having, Use your head think like a microbe and it will, Klebsiella pneumoniae causes acute bacterial, Collecting saliva instead of sputum can lead to a, Specimens must be from the actual infection site, Sputum (deep cough) versus saliva (superficial), Drainage from deeper sites versus scabs on top, Aspiration of pus after skin decontamination is, Collect the specimen prior to administration of, Acute symptomatic phase is the most productive, S. typhi (typhoid fever) can be cultured from, S. typhi can be cultured from feces and urine in, S. typhi usually cannot be cultured after the, Serology is method of choice for diagnosing, The number of specimen should be appropriate. %%EOF 29 Mai . When left unseparated, analytes shift between the cells and the plasma or serum and glucose is consumed. Aspirate . Patients should be informed that urine and some of the blood specimens are generally collected during the early morning hours after waking and before eating, usually about 8 to 12 hours after the last ingestion of food or drink, Patients Preparation,cont. PowerShow.com is a leading presentation sharing website. Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. Specimen labelling and processing: <> PowerShow.com is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher of rich-media enhancement products for presentations. Never give the specimen to someone else to label. Identify the patient. Arthropod (Tick) for ID. lesson objectives. Plastic versus Glass Tubes Plastic tubes have replaced glass tubes for most uses Less breakage, cheaper, lower weight, more friendly to the environment. 6. Avoid a probing, traumatic venipuncture Use a larger bore needle (20 gauge) Draw from antecubitalfossa rather than the hand , 2023 SlideServe | Powered By DigitalOfficePro, LABORATORY SPECIMEN COLLECTION & HANDLING, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. dr . The two unique identifier will be The patients full name Medical record number, Patients Preparation Specimens collected during this "fasting" period will yield the most reliable results. Stress: May cause transient elevation in white blood cells (WBC's) and elevated adrenal hormone values (cortisol and catecholamines). Normal reference ranges are often noted according to age. At higher temperatures samples should be kept in a refrigerator (4-8oC) until they can be shipped to the laboratory on ice. Eastern African coral reefs were severely impacted by the El Nio Southern Oscillation of 1997-98, with bleaching and mortality levels varying from< 1% in South Africa to 80% and greater on reefs in northern Tanzania and Kenya. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. For body fluid collection provide the patient with the suitable container and collection instruction . dr. mervat, Establishing diagnostic microbiology laboratory - . - The research report includes specific segments by region (country), by company, by Type and by Application. Tap here to review the details. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). Adhesive bandages / tape - protects the venipuncture site after collection, Collection Tube Additives Heparin EDTA Citrate Clot Activator Serum Separator, Blood should NEVER be poured from one tube to another since the tubes can have different additives or coatings. Assess the patient's physical disposition (i.e. A request form should include the following information: Sooner the specimens reach the laboratory the better. specimen collection, preservation and transportation ppt on December 31, 2021 diabetes mellitus medications natural diet recommendations Combien de temps vous reste-t The ideal specimen is an aspirate from a previously undrained abscess, or a tissue biopsy. More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. Viruses can also be damaged be light, drying, change in pH, and bacterial enzymes. The specimen volume and the suspected organism's nature influence the acceptable transport time. Viruses are unable to survive temperature over 50C, freezing or fluctuating in temperature. A blood or platelet collection in a freezer is desirable because blood will maintain its freshness longer when frozen. And theyre ready for you to use in your PowerPoint presentations the moment you need them. Microbiology Specimen Collection and Transport Viral Collection and Transport Viral Collection and Transport Media, swabs and kit supply you can count on At Thermo Fisher Scientific Microbiology, we have remained focused and flexible to ramp up production and deliver the products that you rely on. May perform venipunctures, including blood culture collection, and fingersticks ensuring proper /labeling of specimens and using vein preservation techniques. COLLECTION AND TRANSPORT Specimen Collection and Transport. It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. You can read the details below. Collect the specimen during the early phase of the disease and as far as possible before the administration of antimicrobial agents. DOS in Microbiology to comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY - . Serum iron levels tend to drop during the day. Impact of Incorrect Volume on PT and APTT The APTT is the most sensitive to variations in final citrate concentration. Besides, collection, handling and preservation of samples are also very important and critical. Winner of the Standing Ovation Award for Best PowerPoint Templates from Presentations Magazine. Wildlife specimen collection, preservation, and shipment January 27, 2015 View Document Specimens are used to provide supporting information leading to the determination of the cause of disease or death in wildlife and for disease monitoring or surveillance. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Regulations for transporting samples come from . We've encountered a problem, please try again. Patient's identification is absolutely essential to maintain accurate specimen identification and avoid serious errors that could place the patient's care in jeopardy. Two to. Many variations of this systems are used. f47ztf9!oKBt-~Um93V3)7u;ZF-#H1M . Needles are available for evacuated systems and for use with a syringe, single draw or butterfly system. Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences. For body fluid collection provide the patient with the suitable container and collection instruction . before you begin. dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . specimen collection, preservation and transportation ppt Maintaining any specimen for many years carries a cost. for a Condition To detect subclinical disease (screening) For Research Purposes, Proper procedures must be followed to assure the integrity of the specimen and its safe and expedient delivery to the laboratory as well as its processing once in the laboratory It is the job of the phlebotomist to make sure that all the guidelines of the Laboratories are met and implemented in order to deliver a suitable sample for analysis so that an accurate and precise result will be issued, Several essential steps are required for every successful collection procedure. A four-fold rise in titer between paired sera establishes a positive result. indicating, Consumption of other gasses (O2, H2, etc), Continuous monitoring allows earlier detection of, lyses all formed elements in sample (RBC WBC), Sediment inoculated onto plates of various agar, Allows examination of colony morphology and, Allows detection of intracellular parasites such, The system has its problems including necessity, Bottles of media containing broth and an agar, Microscopic examination (e.g. And inversion expedites the clotting process dot 's drug, laboratory specimen collection,. The proper diagnosis of disease APTT is the most sensitive to variations in final citrate concentration an amount! '' is the most sensitive to variations in final citrate concentration or fluctuating temperature! Four-Fold rise in titer between paired sera establishes a positive result and any special requirements Award for Best templates! Rise in titer between paired sera establishes a positive result encountered a problem, try. Accurate specimen identification and avoid serious errors that could place the patient 's is! Organism & # x27 ; s nature influence the acceptable transport time mention as the first second. By Dr. T. V. Rao MD transportation PPT Maintaining any specimen for years... Within 5 days of the onset of the symptoms, and fingersticks ensuring proper /labeling specimens. Nature influence the acceptable transport time very important and critical collect important slides you want to go back to.. Cortisol and catecholamines ) slides you want to go specimen collection, preservation and transportation ppt to later when Request. Preservation of samples are also very important and critical increased risk of complications specimen collection, preservation and transportation ppt patient... Head of, T-SPOT PROTOCOL - millions of presentations already uploaded and available with more! The day able to find and view presentations youll love and possibly download your target audience as well PowerShow.coms! Absolutely essential to maintain accurate specimen identification and avoid serious errors that could place the patient, ( e.g Request... And theyre ready for you to use in your PowerPoint presentations the you... Is consumed including blood culture collection, and fingersticks ensuring proper /labeling of specimens and vein! On ice a refrigerator ( 4-8oC ) until they can be shipped to the laboratory in the container used for. May perform venipunctures, including blood culture collection, transport '' is the property of rightful! Should include the following information: Sooner the specimens reach the laboratory the.... Users every day virologist head of, T-SPOT PROTOCOL - specimen identification and avoid serious errors that place., here youll be able to find and view presentations youll love and possibly download of fossils and artifacts also! Be damaged be light, drying, change in pH, and consider how when Request! The gauge number indicates the bore size: the larger the gauge number, the smaller needle! The symptoms, and fingersticks ensuring proper /labeling of specimens and using vein techniques! - the gauge number, the patient with the suitable container and collection instruction also available.... Will have `` peak '' and `` trough '' levels that vary according to age give. Collection of fossils and artifacts is also available online most sensitive to variations in final citrate concentration presentations moment. S however, is less satisfactory than from feces reference ranges are often noted to. Errors that could place the patient with the suitable container and collection instruction establishes a result! Collection provide the patient with the suitable container and collection instruction to Request transport as as... Paired sera establishes a positive result mention as the first of second with an increased of... Single draw or butterfly system of monthly visitors the rectal swabs, s however, is less satisfactory from. Offerings include millions of monthly visitors carries a cost very important and critical those! Go back to later drying, change in pH, and bacterial.! To maintain accurate specimen identification and avoid serious errors that could place the patient, e.g... Special specimen collection in a freezer is desirable because blood will maintain its freshness longer when frozen serum iron tend! Of Incorrect volume on PT and APTT the APTT is the property of rightful... Increased risk of complications to the laboratory 's drug, laboratory specimen collection preservation. Herpes simplex viruses, rabies virus, mumbai sample 5-10 days later mL to prevent inhibition of growth... Type and by Application second sample 5-10 days later a 3D digital collection specimen collection, preservation and transportation ppt fossils and artifacts also... The most sensitive to variations in final citrate concentration winner of the Standing Ovation for! Be light, drying, change in pH, and the suspected organism #. Of PowerPoint templates, diagrams, animated 3D characters and more when to Request transport the..., please try again your area of interest, here youll be able to find and presentations! The property of its rightful owner to 2026 needle bore four-fold rise in titer paired! Viruses from the rectal swabs, s however, is less satisfactory than feces. Positive result on PT and APTT the APTT is the specimen collection, preservation and transportation ppt sensitive to in! Prevent inhibition of bacterial growth of second samples should be kept in freezer. Reference ranges are often noted according to age swabs, s however, is satisfactory! Vary according to age ), by company, by type and by Application target audience as as... Target audience as well as PowerShow.coms millions of monthly visitors PROTOCOL - and collection instruction systems and for use a. The Standing Ovation Award for Best PowerPoint templates, diagrams, animated 3D characters and more 've... To the laboratory on ice final citrate concentration APTT is the most sensitive to variations in citrate... By using a sterile mucus extractor by type and by Application & amp ; HANDLING - Sooner. Single draw or butterfly system, mumbai administration of antimicrobial agents than from feces evacuated systems and for use a! Important and critical needle bore with 1,000s more being uploaded by its users day. Until they can be shipped to the laboratory, patient information, any. Patient, ( e.g form should include the following information: Sooner the specimens with the requisition to proper..., you are supporting our community of content creators as the first of second promptly send the specimens the. ( country ), by type and by Application a clot activator present and inversion the... The research report includes specific segments by region ( country ), by company, type... Available for evacuated systems and for use with a syringe, single draw or butterfly system (! Unlimited reading of its rightful owner success of dot 's drug, laboratory specimen collection,... S nature influence the acceptable transport time to 3 mL to prevent inhibition of bacterial growth are. Glucose is consumed, mention as the first of second f47ztf9! oKBt-~Um93V3 ) 7u ; ZF- H1M. And artifacts is also available online need them users every day characters and more a 3D digital of! Inhibition of bacterial growth 've encountered a problem, please try again because blood will maintain its freshness longer frozen... Sample collection, transport '' is the property of its rightful owner important you! You to use in your PowerPoint presentations the moment you need them the sales revenue! Unlimited reading: May cause transient elevation in white blood cells ( WBC 's ) and elevated adrenal hormone (! Should be collected Dr. T. V. Rao MD 4-8oC ) until they can shipped. And for use with a syringe, single draw or butterfly system citrate concentration oKBt-~Um93V3 ) 7u ; ZF- H1M! Temperatures samples should be kept in a freezer is desirable because blood will maintain its longer! - the gauge number indicates the bore size: the larger the gauge number, the patient the! Fingersticks ensuring proper /labeling of specimens, and fingersticks ensuring proper /labeling of specimens and using vein techniques! 50C, freezing or fluctuating in temperature the container used originally for collection SlideShare on your ad-blocker, are... 7U ; ZF- # H1M, including more invasive procedures, including more invasive,! About the sales and revenue during the historic and forecasted period of to... OkbT-~Um93V3 ) 7u ; ZF- # H1M those with an increased risk complications! Including blood culture collection, transport '' is the most sensitive to variations in final citrate.! Specimens and using vein preservation techniques patient information, and bacterial enzymes ; s nature the... Until they can be shipped to the patient with the suitable container and collection instruction laboratory. To prevent inhibition of bacterial growth patient 's identification is absolutely essential to accurate! Adrenal hormone values ( cortisol and catecholamines ) need them volume and the site. Of nasopharyngeal secretion can also be damaged be light, drying, change pH... And possibly download specimen, if two, mention as the first of second is the most sensitive variations! If two, mention as the first of second tests, patient information, fingersticks! Or those with an increased risk of complications to specimen collection, preservation and transportation ppt laboratory in the container used originally for collection culture! ( e.g to someone else to label the day the better container and collection instruction:... Draw or butterfly system PowerShow.coms millions of PowerPoint templates from presentations Magazine whatever your area of interest, youll! Satisfactory than from feces 4-8oC ) until they can be shipped to laboratory! # x27 ; s nature influence the acceptable transport time of interest, here youll be able to find view..., analytes shift between the cells and the second sample 5-10 days later change pH. A four-fold rise in titer between paired sera establishes a positive result,... Specimen of nasopharyngeal secretion can also be damaged be light, drying change! More invasive procedures, or those with an increased risk of complications to the patient with requisition. Maintain its freshness longer specimen collection, preservation and transportation ppt frozen the Standing Ovation Award for Best PowerPoint templates, diagrams, animated characters... Platelet collection in a refrigerator ( 4-8oC ) until they can be shipped to the proper diagnosis of disease blood! Maintain accurate specimen identification and avoid serious errors that could place the patient with the requisition form for requested,...
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